Molecular analysis of human adenoviral keratoconjunctivitis cases: Results of a 2-year survey

Objective: This study aimed to determine the adenovirus genotypes and their epidemiological features between January 2018 and
 November 2019, in Istanbul, Turkey.
 Material Methods: Conjunctival swab samples were obtained from patients who clinically diagnosed with keratoconjunctivitis.
 Samples screened an Adeno Detector kit (Rapid Pathogen Screening, RPS Inc., South Williamsport, PA). Nucleic acid
 extraction amplification performed ADENOVIRUS ELITe MGB® In Genius instrument (Elitech
 Group, Torino, Italy). For subtyping of strains, sequencing primers targeted ‘Hypervariable Region 7’ (HVR-7) hexon
 gene used. DNA sequence analysis (n:72) was ABI PRISM® 3100 Genetic Analyzer (Applied Biosystems, USA),
 done by BLAST analysis.
 Results: The median viral load (n: 77) 7 log10 copies/mL (IQR: 4.5-7.4 copies/mL). clinical finding score
 found be significantly higher high group (Adenovirus DNA≥6 Log 10 copies/mL) than low load